Olsen: 477

Loxahatchee National Wildlife Refuge (LNWR)
FIU Station: 365

Panel: OverSample	Season: Spring
BioChem Date/Time: 2005-05-13 16:30:00
BioChem Crew: Mel Parsons/Anne-Marie Hoffman
Plants Date: 2005-05-20	Aquatic Date:

Location		NAD83	NAD27
Latitude	26.408667	26.408665
Longitude	-80.3783	-80.378299
UTM Y (northing)		2920888	2921092
UTM X (easting)		561981	562002

Comments: MARIO ONLY. DEEP WATER. DUE TO WATER DEPTH AND UNCONSOLIDATED ORGANIC DETRITIS, CORES WERE TAKEN DEEPER THAN 10 CM AND THE TOP 10 CM EXTRUDED OUT.

Basic

Item	Value	Description
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Camera	NEPA	Digital camera used for field documentation of habitat and soil core
CHLAVOL	140	volume of surface water filtered for chlorophyll a analysis
Chopper	2	number of helicopter and associated sampling equipment and supply cache
COND	442	in situ surface water conductivity, YSI sonde
Cycle	6	sampling cycle number
DO	5.55	in situ surface water DO, YSI sonde
FISHNO1	17	number of mosquitofish collected for Hg analysis
FISHNO2	15	number of mosquitofish collected for isotope analysis
FLOCCOLperc	100	Percent of floc collected from core
FLOCCONT	1 tub	number and kind of container used for floc
Flow	sheetflow	description of surface water flow at sampling site
PERIPB	no	benthic periphyton collected
PERIPE	no	epiphytic periphyton collected
PERIPF	no	floating mat periphyton collected
pH	7.33	in situ surface water pH, YSI sonde
Soiltype	peat	soil type categories, by visual observation
Station	365	sampling station numbers, unique for 2005, assigned by EPA to the Olsen site numbers; many of the FIU researchers use these numbers as their sample identifiers
Temp	28.02	in situ surface water temperature, YSI sonde
Turb	0.4	in situ surface water turbidity, YSI sonde
VEGTYPE	sawgrass marsh	plant community observed at sampling site
Weather	1 clear	weather conditions
X	OV NOM	nominal = sampled within 5 meters of GPS coordinates shifted = 5-20 meters station rejected = >20 meters
YSIDEPTH	6	depth of deployment of probe array in surface water
PlantDate	2005-05-20	Plant collection date

Item	Value 1	Value 2	Value 3	Average	Units	Description
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PWEAgCl	-120	-129	-124	-124.33	mV	pore water, field-measured, in-line Ag/AgCl reference microprobe
PWEh	79	70	75	74.7	mV	pore water, field-measured, in-line Ag/AgCl reference microprobe corrected to standard Hydrogen electrode by adding 199 mV
SoilCTH	10	10	10	10	cm	vertical extent (length) of soil sample, from soil surface to bottom of core
SoilTHI	9.5	9.5	9.4	9.5	feet	soil thickness, metal probe to point of refusal
WatDept	2.5	2.4	2.5	2.5	feet	water depth

BioChemistry

Item	Value	Units	Q	Protocol	Description
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AFDWFCFS	0.91	g/g		FIU SERC	Ash Free Dry Weight of floc
AFDWSDFS	91.15	%		FIU SERC	Ash Free Dry Weight for soil
APASWEE	0.43	µmole/L*hr		EPA ESAT	Alkaline Phosphatase Activity in surface water
BDFCFS	0.014	g/cc		FIU SERC	Bulk Density of floc
BDSDFS	0.062	g/cc		FIU SERC	Bulk Density for soil
BRPWEA	0.11	mg/L		EPA SESD	Bromide in pore water
BRSWEA	0.1	mg/L	U	EPA SESD	Bromide in surface water
CHLAFCFS	29.258	µg/g		FIU SERC	chlorophyll a in floc, dry weight
CHLASWFB	2.4	µg/L		FIU SERC	Chlorophyll a in surface water
CLPWEA	54	mg/L		EPA SESD	Chloride in pore water
CLSWEA	51	mg/L		EPA SESD	Chloride in surface water
DOCSWD	19	mg/L		FDEP	Dissolved Organic Carbon in surface water
FDOCPWD	26	mg/L		FDEP	Dissolved Organic Carbon in porewater
FNH4RPWF	0.51	mg/L		FIU SERC	Filtered Ammonia in pore water, recalculated
FNH4RSWF	0.0094	mg/L	J	FIU SERC	Filtered Ammonia in surface water, recalculated
FNNRPWFB	0.083	mg/L		FIU SERC	Filtered Nitrite + Nitrate in pore water, recalculated
FNNRSWFB	0.045	mg/L		FIU SERC	Filtered Nitrite + Nitrate in surface water, recalculated
FNO2RPWF	0.0072	mg/L	U	FIU SERC	Filtered Nitrite in pore water, recalculated
FNO2RSWF	0.0005	mg/L	J	FIU SERC	Filtered Nitrite in surface water, recalculated
FNO3RPWF	0.076	mg/L	J	FIU SERC	Filtered Nitrate in pore water, recalculated
FNO3RSWF	0.045	mg/L	J	FIU SERC	Filtered Nitrate in surface water, recalculated
FPWEA	0.17	mg/L		EPA SESD	Fluoride in pore water
FSWEA	0.19	mg/L		EPA SESD	Fluoride in surface water
H2SPWEE	3.9	mg/L		EPA ESAT	Sulfide in pore water
H2SSWEE	0.02	mg/L	U	EPA ESAT	Sulfide in surface water
MCFCFS	0.088	g/g		FIU SERC	Mineral Content of floc
MCSDFS	0.088	g/g		FIU SERC	Mineral Content of soil
MEHGFCFC	0.9	ng/g	J	FIU SERC	Methyl Mercury in floc
MEHGSDFC	0.46	ng/g	J	FIU SERC	Methyl Mercury in soil
MEHGSWB	0.24	ng/L		Battelle	Methyl Mercury in surface water
pHSDEE	7			EPA SESD	ex situ soil pH
SO4PWEA	1.1	mg/L		EPA SESD	Sulfate in pore water
SO4SWEA	9	mg/L		EPA SESD	Sulfate in surface water
SRPRPWFB	0.0409	mg/L		FIU SERC	Soluble Reactive Phosphorus in pore water, recalculated
SRPRSWFB	3	µg/L	J	FIU SERC	Soluble Reactive Phosphorus in surface water, recalculated
TCFCFB	42	%		FIU SERC	Total Carbon in floc
TCSDFB	47	%		FIU SERC	Total Carbon in soil
THGFCFC	110	ng/g	J	FIU SERC	Total Mercury in floc
THGFSFC	17	µg/kg		FIU SERC	Total Mercury in mosquitofish (Gambusia affinis), average of 7 individuals
THGFSSD	1.3	µg/kg			standard devaition of total mercury in individual mosquitofish for 7 individuals
THGSDFC	220	ng/g	J	FIU SERC	Total Mercury in soil
THGSWD	1.6	ng/L	U	FDEP	Total Mercury in surface water
TINAPWFB	0.59	mg/L		FIU SERC	Total Inorganic Nitrogen (calculated) in pore water, recalculated
TINSWFB	0.055	mg/L	J	FIU SERC	Total Inorganic Nitrogen (calculated) in surface water, recalculated
TNFCFB	3.4	%		FIU SERC	Total Nitrogen in floc
TNRSWFB	0.25	mg/L	U	FIU SERC	Total Nitrogen in surface water, recalculated
TNSDFB	2.9	%		FIU SERC	Total Nitrogen in soil
TONSWFB	0.19	mg/L	J	FIU SERC	Total Organic Nitrogen (calculated) in surface water, recalculated
TPRFCFB	770	µg/g		FIU SERC	Total Phosphorus in floc, recalculated
TPRSDFB	460	µg/g		FIU SERC	Total Phosphorus in soil, recalculated
TPRSWFB	8.3	µg/L		FIU SERC	Total Phosphorus in surface water, recalculated
WCFCFS	0.99	g/g		FIU SERC	Water Content of floc
WCSDFS	0.94	g/g		FIU SERC	Water Content of soil

Plants

All sites sampled had 1 transect that ran south to north, with the southern end sampled 5 m due west of the biogeochemical sampling point. If a second “habitat type”, as based on visual assessment of the botanist in the crew, was located within a 50 m radius of the first sampling point, a second transect was done in that habitat.

Left Panorama

Right Panorama

Transects

The number in each plant column records the number of quarter-meter-squared quadrats in 5 (1-meter-squared) quadrats in which that species was found at any given site.The 5 (1-meter-squared) quadrats were distributed to the right and left every 2 m along the 10 m transect.If the plant was not present, the cell has a 0;if the plant was present in every quarter-meter-squared quadrat, the number in the cell is 20.

Item	Transect 1	Transect 2	Family	Scientific Name	Common Name
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CladJama	5	19	Cyperaceae	Cladium jamaicense	Saw-grass, Jamaica swamp sawgrass
UtriFoli	10	0	Lentibulariaceae	Utricularia foliosa	Leafy bladderwort
UtriGibb	13	6	Lentibulariaceae	Utricularia gibba	Cone-spur bladderwort, Humped bladderwort
UtriPurp	19	14	Lentibulariaceae	Utricularia purpurea	Eastern purple bladderwort
NympOdor	12	0	Nymphaeaceae	Nymphaea odorata	American white waterlily
PontCord	0	1	Pontederiaceae	Pontederia cordata	Pickerelweed
TyphDomi	0	1	Typhaceae	Typha domingensis	Southern cat-tail

Exotics

In the 2005 R-EMAP sampling invasive exotic plant species were surveyed in two ways. The first survey was done on the helicopter fly-in to the GPS site and covered the general area of the site, while the second was a 360° site survey from the helicopter pontoons after landing and covered the immediate area of the site. In the aerial (A) helicopter survey we looked for the presence of six invasive exotic species (Casuarina spp., Lygodium microphyllum, Melaleuca quinquenervia, Neyraudia reynaudiana, Pennisetum purpureum and Schinus terebinthefolius), as well as the presence of cattail (Typha domingensis). In the survey from the helicopter floats (F) we recorded the presence of any exotic seen; the only additional exotic species observed from the floats was Salvinia minima. In both surveys we recorded the presence of species and quantified their abundance using categories for the number of individuals. The categories used were 0 (i.e., not present), 1 individual, 2 - 10 individuals, and > 10. We did not find Neyraudia reynaudiana at any site, so this species was not included in the database.

Item	Abundance	Scientific Name	Source
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CasuSp_A	0	Casuarina species	aerial fly-in
CasuSp_F	0	Casuarina species	floats
LygoMicr_A	0	Lygodium microphyllum	aerial fly-in
LygoMicr_F	0	Lygodium microphyllum	floats
MelaQuin_A	0	Melaleuca quinquenervia	aerial fly-in
MelaQuin_F	0	Melaleuca quinquenervia	floats
PennPurp_A	0	Pennisetum purpureum	aerial fly-in
PennPurp_F	0	Pennisetum purpureum	floats
SalvMini_F	0	Salvinia minima	floats
SchiTere_A	0	Schinus terebinthefolius	aerial fly-in
TyphDomi_A	> 10	Typha domingensis	aerial fly-in
TyphDomi_F	> 10	Typha domingensis	floats

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