Station FLE04524-463

Olsen: 463

Loxahatchee National Wildlife Refuge (LNWR)
FIU Station: 242

Panel: RegularSeason: Fall
BioChem Date/Time: 2005-11-15 10:50:00
BioChem Crew: Sara Taich/Bobby Lewis
Plants Date: 2005-12-06Aquatic Date: 2005-11-29

SB SB
Location NAD83NAD27
Latitude26.4865714526.486557 
Longitude-80.322313-80.322304 
UTM Y (northing) 29295432929747
UTM X (easting) 567520567541

Comments: bottom kicked up when landed (open water). Bobby had to jump in and hold chopper. Had to use anchor. New C-N filters used starteing with pw (sw used old filters) lot #00162m23

Basic

ItemValueDescription
Camera2Digital camera used for field documentation of habitat and soil core
CHLAVOL100volume of surface water filtered for chlorophyll a analysis
Chopper2number of helicopter and associated sampling equipment and supply cache
COND107in situ surface water conductivity, YSI sonde
Cycle7sampling cycle number
DO4.78in situ surface water DO, YSI sonde
FISHNO115number of mosquitofish collected for Hg analysis
FLOCCOLperc100Percent of floc collected from core
FLOCCONT1 tubnumber and kind of container used for floc
Flowsheetflowdescription of surface water flow at sampling site
PERIPBnobenthic periphyton collected
PERIPEnoepiphytic periphyton collected
PERIPFnofloating mat periphyton collected
pH5.71in situ surface water pH, YSI sonde
Soiltypepeatsoil type categories, by visual observation
Station242sampling station numbers, unique for 2005, assigned by EPA to the Olsen site numbers; many of the FIU researchers use these numbers as their sample identifiers
Temp24.23in situ surface water temperature, YSI sonde
Turb2.5in situ surface water turbidity, YSI sonde
VEGTYPEwet prairieplant community observed at sampling site
Weather2 lt overcastweather conditions
XNOMINALnominal = sampled within 5 meters of GPS coordinates
shifted = 5-20 meters
station rejected = >20 meters
YSIDEPTH6depth of deployment of probe array in surface water
PlantDate2005-12-06Plant collection date

ItemValue 1Value 2Value 3AverageUnitsDescription
DEPBEDR9.89.79.79.73feetdepth to bedrock, point of refusal with metal probe
FLOCTH24162220.7cmvertical extent (length) of floc layer in core
PWEAgCl23.942.158.741.57mVpore water, field-measured, in-line Ag/AgCl reference microprobe
PWEh222.9241.1257.7240.6mVpore water, field-measured, in-line Ag/AgCl reference microprobe corrected to standard Hydrogen electrode by adding 199 mV
SoilCTH10101010cmvertical extent (length) of soil sample, from soil surface to bottom of core
SoilTHI87.97.87.9feetsoil thickness, metal probe to point of refusal
WatDept1.81.81.91.83feetwater depth

BioChemistry

A A G SC
ItemValueUnitsQProtocolDescription
AFDWFCFS0.95g/gFIU SERCAsh Free Dry Weight of floc
AFDWSDFS96.24%FIU SERCAsh Free Dry Weight for soil
APASWEE0.67µmole/L*hrEPA ESATAlkaline Phosphatase Activity in surface water
AVSSDA32mg/kgUJAZC Inc.Acid Volatile Sulfide in soil
BDFCFS0.016g/ccFIU SERCBulk Density of floc
BDSDFS0.064g/cc FIU SERCBulk Density for soil
BRPWEA0.1mg/LUEPA SESDBromide in pore water
BRSWEA0.1mg/LUEPA SESDBromide in surface water
CHLAFCFS13.779µg/gFIU SERCchlorophyll a in floc, dry weight
CHLASWFB3.9µg/LFIU SERCChlorophyll a in surface water
CLPWEA21mg/LEPA SESDChloride in pore water
CLSWEA18mg/LEPA SESDChloride in surface water
DOCSWD19mg/LFDEPDissolved Organic Carbon in surface water
FDOCPWD38mg/LFDEPDissolved Organic Carbon in porewater
FNH4RPWF0.087mg/LFIU SERCFiltered Ammonia in pore water, recalculated
FNH4RSWF0.038mg/LFIU SERCFiltered Ammonia in surface water, recalculated
FNNRPWFB0.22mg/LFIU SERCFiltered Nitrite + Nitrate in pore water, recalculated
FNNRSWFB0.037mg/LFIU SERCFiltered Nitrite + Nitrate in surface water, recalculated
FNO2RPWF0.0058mg/LFIU SERCFiltered Nitrite in pore water, recalculated
FNO2RSWF0.0023mg/LFIU SERCFiltered Nitrite in surface water, recalculated
FNO3RPWF0.21mg/LFIU SERCFiltered Nitrate in pore water, recalculated
FNO3RSWF0.035mg/LFIU SERCFiltered Nitrate in surface water, recalculated
FPWEA0.68mg/LEPA SESDFluoride in pore water
FSWEA0.05mg/LUEPA SESDFluoride in surface water
H2SPWEE0.052mg/LJEPA ESATSulfide in pore water
H2SSWEE0.02mg/LUEPA ESATSulfide in surface water
MCFCFS0.05g/g FIU SERCMineral Content of floc
MCSDFS0.038g/gJFIU SERCMineral Content of soil
MEHGFCFC3.4ng/gFIU SERCMethyl Mercury in floc
MEHGSDFC2.7ng/gFIU SERCMethyl Mercury in soil
MEHGSWB0.13ng/LAJBattelleMethyl Mercury in surface water
pHSDEE5.32 EPA SESDex situ soil pH
SO4PWEA0.092mg/LJEPA SESDSulfate in pore water
SO4SWEA0.031mg/LJEPA SESDSulfate in surface water
SRPRPWFB0.0092mg/LFIU SERCSoluble Reactive Phosphorus in pore water, recalculated
SRPRSWFB3.8µg/LJFIU SERCSoluble Reactive Phosphorus in surface water, recalculated
TCFCFB49% FIU SERCTotal Carbon in floc
TCSDFB52% FIU SERCTotal Carbon in soil
THGFCFC160ng/gFIU SERCTotal Mercury in floc
THGFSFC23µg/kgJFIU SERCTotal Mercury in mosquitofish (Gambusia affinis), average of 7 individuals
THGFSSD4µg/kg standard devaition of total mercury in individual mosquitofish for 7 individuals
THGSDFC190ng/gFIU SERCTotal Mercury in soil
THGSWD3.6ng/LFDEPTotal Mercury in surface water
TINAPWFB0.31mg/LFIU SERCTotal Inorganic Nitrogen (calculated) in pore water, recalculated
TINSWFB0.077mg/LFIU SERCTotal Inorganic Nitrogen (calculated) in surface water, recalculated
TNFCFB4.5% FIU SERCTotal Nitrogen in floc
TNRSWFB0.6mg/LFIU SERCTotal Nitrogen in surface water, recalculated
TNSDFB3.9% FIU SERCTotal Nitrogen in soil
TONSWFB0.53mg/LFIU SERCTotal Organic Nitrogen (calculated) in surface water, recalculated
TPRFCFB690µg/gFIU SERCTotal Phosphorus in floc, recalculated
TPRSDFB330µg/gFIU SERCTotal Phosphorus in soil, recalculated
TPRSWFB8.8µg/LFIU SERCTotal Phosphorus in surface water, recalculated
WCFCFS0.98g/gFIU SERCWater Content of floc
WCSDFS0.94g/g FIU SERCWater Content of soil

Plants

A A

All sites sampled had 1 transect that ran south to north, with the southern end sampled 5 m due west of the biogeochemical sampling point. If a second “habitat type”, as based on visual assessment of the botanist in the crew, was located within a 50 m radius of the first sampling point, a second transect was done in that habitat.

Left Panorama

LP3 LP2 LP1

Right Panorama

RP1 RP2 RP3

Transects

PT

The number in each plant column records the number of quarter-meter-squared quadrats in 5 (1-meter-squared) quadrats in which that species was found at any given site.The 5 (1-meter-squared) quadrats were distributed to the right and left every 2 m along the 10 m transect.If the plant was not present, the cell has a 0;if the plant was present in every quarter-meter-squared quadrat, the number in the cell is 20.

ItemTransect 1Transect 2FamilyScientific NameCommon Name
SagiLanc1AlismataceaeSagittaria lancifoliaBulltongue arrowhead, Lanceleaf arrowhead
EleoElon17CyperaceaeEleocharis elongataSlim spikerush
FuirScir7CyperaceaeFuirena scirpoideaSouthern umbrellasedge
UtriFoli6LentibulariaceaeUtricularia foliosaLeafy bladderwort
UtriGibb6LentibulariaceaeUtricularia gibbaCone-spur bladderwort, Humped bladderwort
UtriPurp16LentibulariaceaeUtricularia purpureaEastern purple bladderwort
NympAqua5MenyanthaceaeNymphoides aquaticaBig floatingheart
NympOdor18NymphaeaceaeNymphaea odorataAmerican white waterlily
PaniHemi7PoaceaePanicum hemitomonMaidencane

Exotics

In the 2005 R-EMAP sampling invasive exotic plant species were surveyed in two ways. The first survey was done on the helicopter fly-in to the GPS site and covered the general area of the site, while the second was a 360° site survey from the helicopter pontoons after landing and covered the immediate area of the site. In the aerial (A) helicopter survey we looked for the presence of six invasive exotic species (Casuarina spp., Lygodium microphyllum, Melaleuca quinquenervia, Neyraudia reynaudiana, Pennisetum purpureum and Schinus terebinthefolius), as well as the presence of cattail (Typha domingensis). In the survey from the helicopter floats (F) we recorded the presence of any exotic seen; the only additional exotic species observed from the floats was Salvinia minima. In both surveys we recorded the presence of species and quantified their abundance using categories for the number of individuals. The categories used were 0 (i.e., not present), 1 individual, 2 - 10 individuals, and > 10. We did not find Neyraudia reynaudiana at any site, so this species was not included in the database.

ItemAbundanceScientific NameSource
CasuSp_A0Casuarina speciesaerial fly-in
CasuSp_F0Casuarina speciesfloats
LygoMicr_A2 - 10Lygodium microphyllumaerial fly-in
LygoMicr_F2 - 10Lygodium microphyllumfloats
MelaQuin_A> 10Melaleuca quinquenerviaaerial fly-in
MelaQuin_F1Melaleuca quinquenerviafloats
PennPurp_A0Pennisetum purpureumaerial fly-in
PennPurp_F0Pennisetum purpureumfloats
SalvMini_F0Salvinia minimafloats
SchiTere_A0Schinus terebinthefoliusaerial fly-in
TyphDomi_A0Typha domingensisaerial fly-in
TyphDomi_F0Typha domingensisfloats

Vegetation Map

Aquatic

As part of the EPA‘s R-EMAP Phase III wet season sampling, 54 sites (designated by Olsen numbers/station numbers) spanning the Greater Everglades were sampled for aquatic fauna in November and December 2005. Densities of small fish and large macroinvertebrates were quanitifed using a standardized throw-trap methodology, and macroinvertebrate infauna were enumerated from periphyton cores and floc samples. Community compositions and wet weights were recorded for throw-trap samples, and fish and crayfish lengths were measured. Counts and community compositions were recorded for infauna and scaled according to substrate samples (g AFDM and/or mL of periphyton or floc). Finally, stable isotope analysis was conducted on a subset of the fauna. These data were then used to address questions about consumer densities, community structure, and food webs along hydrological (disturbance) and productivity gradients.

Three throw-traps replicates were deployed per station.

TypeItemThrowtrap 1Throwtrap 2Throwtrap 3UnitsScientific NameCommon NameNote
PeriVolume700800800mlbiovolume of periphyton and submerged plants
PlantCover202030%percent of water surface covered by vegetation
PlantHt221925cmplant height from water surface
WaterDepth554842cm
Fish0TotalFish351063
Fishelaeve124Elassoma evergladeiEverglades pygmy sunfish
Fishfunchr204Fundulus chrysotusGolden topminnow
Fishgamhol518Gambusia holbrookiMosquitofish
Fishhetfor16645Heterandria formosaLeast killifish
Fishjorflo001Jordanella floridaeFlagfish
Fishlepmar100Lepomis marginatusDollar sunfish
Fishlucgoo1011Lucania goodeiBluefin killifish
Invertebrates0TotalInv261858
Inverterbatescelepo316Celithemis eponinaDragonfly larva
Inverterbatescoenag100family CoenagrionidaeDamselfly larvae
Inverterbatescoring001Coryphaeschna ingensDragonfly larva
Inverterbateslibnee001Libellula needhamiDragonfly larva
Inverterbatesnotvir011Notophthalmus viridescensPeninsula newt
Inverterbatespalpal191235Palaemonetes paludosusGrass shrimp
Inverterbatespelfem002Pelocoris femoratusAlligator flea
InverterbatesprofalF004Procambarus fallax FCrayfish (female)
InverterbatesprofalM012Procambarus fallax MCrayfish (male)
Inverterbatesprospp326Procambarus sppCrayfish unknown species
Inverterbatesranatp010Rana spp. tp.Pig frog tadpole/Leopard frog tadpole
Vegetationelecel0035stem countsEleocharis cellulosaSpikerush
Vegetationeleelo02067stem countsEleocharis elongataSpikerush
Vegetationnymodo152712stem countsNymphaea odoratafragrant water-lily
Vegetationpanhem12817stem countsPanicum hemitomonmaidencane
Vegetationutrpur1001000stem countsUtricularia purpureapurple bladderwort