Station FLE04524-462

Olsen: 462

Loxahatchee National Wildlife Refuge (LNWR)
FIU Station: 244

Panel: RegularSeason: Fall
BioChem Date/Time: 2005-11-15 09:15:00
BioChem Crew: Sara Taich/Bobby Lewis
Plants Date: 2005-12-06Aquatic Date: 2005-11-29

SB SB
Location NAD83NAD27
Latitude26.4985195826.498524 
Longitude-80.382945-80.382943 
UTM Y (northing) 29308382931042
UTM X (easting) 561470561491

Comments: actually within 15 ft, not shifted. Vegetation-mixed. Veg type: 2,5 (sawgrass marsh, willow)

Basic

ItemValueDescription
Camera2Digital camera used for field documentation of habitat and soil core
CHLAVOL140volume of surface water filtered for chlorophyll a analysis
Chopper2number of helicopter and associated sampling equipment and supply cache
COND129in situ surface water conductivity, YSI sonde
Cycle7sampling cycle number
DO1.82in situ surface water DO, YSI sonde
FISHNO115number of mosquitofish collected for Hg analysis
FLOCCOLperc100Percent of floc collected from core
FLOCCONT1 storemorenumber and kind of container used for floc
Flowsheetflowdescription of surface water flow at sampling site
PERIPBnobenthic periphyton collected
PERIPEnoepiphytic periphyton collected
PERIPFnofloating mat periphyton collected
pH5.81in situ surface water pH, YSI sonde
Soiltypepeatsoil type categories, by visual observation
Station244sampling station numbers, unique for 2005, assigned by EPA to the Olsen site numbers; many of the FIU researchers use these numbers as their sample identifiers
Temp22.48in situ surface water temperature, YSI sonde
Turb2.1in situ surface water turbidity, YSI sonde
VEGTYPEsawgrass marshplant community observed at sampling site
Weather2 lt overcastweather conditions
XNOMINALnominal = sampled within 5 meters of GPS coordinates
shifted = 5-20 meters
station rejected = >20 meters
YSIDEPTH6depth of deployment of probe array in surface water
PlantDate2005-12-06Plant collection date

ItemValue 1Value 2Value 3AverageUnitsDescription
DEPBEDR12.31312.112.47feetdepth to bedrock, point of refusal with metal probe
FLOCTH6434.3cmvertical extent (length) of floc layer in core
PWEAgCl-41.6-54.7-51.7-49.33mVpore water, field-measured, in-line Ag/AgCl reference microprobe
PWEh157.4144.3147.3149.7mVpore water, field-measured, in-line Ag/AgCl reference microprobe corrected to standard Hydrogen electrode by adding 199 mV
SoilCTH10101010cmvertical extent (length) of soil sample, from soil surface to bottom of core
SoilTHI11.211.810.9511.32feetsoil thickness, metal probe to point of refusal
WatDept1.11.21.151.15feetwater depth

BioChemistry

A A G G SC
ItemValueUnitsQProtocolDescription
AFDWSDFS93.39%FIU SERCAsh Free Dry Weight for soil
APASWEE0.47µmole/L*hrEPA ESATAlkaline Phosphatase Activity in surface water
BDSDFS0.068g/cc FIU SERCBulk Density for soil
BRPWEA0.1mg/LUEPA SESDBromide in pore water
BRSWEA0.1mg/LUEPA SESDBromide in surface water
CHLASWFB5µg/LFIU SERCChlorophyll a in surface water
CLPWEA20mg/LEPA SESDChloride in pore water
CLSWEA20mg/LEPA SESDChloride in surface water
DOCSWD17mg/LFDEPDissolved Organic Carbon in surface water
FDOCPWD17mg/LFDEPDissolved Organic Carbon in porewater
FNH4RPWF0.67mg/LFIU SERCFiltered Ammonia in pore water, recalculated
FNH4RSWF0.12mg/LFIU SERCFiltered Ammonia in surface water, recalculated
FNNRPWFB0.054mg/LFIU SERCFiltered Nitrite + Nitrate in pore water, recalculated
FNNRSWFB0.074mg/LFIU SERCFiltered Nitrite + Nitrate in surface water, recalculated
FNO2RPWF0.0047mg/LFIU SERCFiltered Nitrite in pore water, recalculated
FNO2RSWF0.0051mg/LFIU SERCFiltered Nitrite in surface water, recalculated
FNO3RPWF0.049mg/LFIU SERCFiltered Nitrate in pore water, recalculated
FNO3RSWF0.068mg/LFIU SERCFiltered Nitrate in surface water, recalculated
FPWEA0.05mg/LUEPA SESDFluoride in pore water
FSWEA0.05mg/LUEPA SESDFluoride in surface water
H2SPWEE0.091mg/LJEPA ESATSulfide in pore water
H2SSWEE0.02mg/LUEPA ESATSulfide in surface water
MCSDFS0.066g/gFIU SERCMineral Content of soil
MEHGFCFC8.4ng/gFIU SERCMethyl Mercury in floc
MEHGSDFC12ng/gFIU SERCMethyl Mercury in soil
MEHGSWB0.22ng/LJBattelleMethyl Mercury in surface water
pHSDEE5.92 EPA SESDex situ soil pH
SO4PWEA0.19mg/LEPA SESDSulfate in pore water
SO4SWEA0.3mg/LEPA SESDSulfate in surface water
SRPRPWFB0.024mg/LFIU SERCSoluble Reactive Phosphorus in pore water, recalculated
SRPRSWFB4.2µg/LJFIU SERCSoluble Reactive Phosphorus in surface water, recalculated
TCSDFB50% FIU SERCTotal Carbon in soil
THGFCFC110ng/gFIU SERCTotal Mercury in floc
THGFSFC88µg/kgFIU SERCTotal Mercury in mosquitofish (Gambusia affinis), average of 7 individuals
THGFSSD35µg/kg standard devaition of total mercury in individual mosquitofish for 7 individuals
THGSDFC140ng/gFIU SERCTotal Mercury in soil
THGSWD4.6ng/LAFDEPTotal Mercury in surface water
TINAPWFB0.73mg/LFIU SERCTotal Inorganic Nitrogen (calculated) in pore water, recalculated
TINSWFB0.201mg/LFIU SERCTotal Inorganic Nitrogen (calculated) in surface water, recalculated
TNRSWFB0.91mg/LFIU SERCTotal Nitrogen in surface water, recalculated
TNSDFB2.7% FIU SERCTotal Nitrogen in soil
TONSWFB0.71mg/LFIU SERCTotal Organic Nitrogen (calculated) in surface water, recalculated
TPRSDFB400µg/gFIU SERCTotal Phosphorus in soil, recalculated
TPRSWFB12.2µg/LFIU SERCTotal Phosphorus in surface water, recalculated
WCSDFS0.94g/g FIU SERCWater Content of soil

Plants

A

All sites sampled had 1 transect that ran south to north, with the southern end sampled 5 m due west of the biogeochemical sampling point. If a second “habitat type”, as based on visual assessment of the botanist in the crew, was located within a 50 m radius of the first sampling point, a second transect was done in that habitat.

Left Panorama

LPX LP5 LP4 LP3 LP2 LP1

Right Panorama

RP1 RP2 RP3 RP4

Transects

PT ST

The number in each plant column records the number of quarter-meter-squared quadrats in 5 (1-meter-squared) quadrats in which that species was found at any given site.The 5 (1-meter-squared) quadrats were distributed to the right and left every 2 m along the 10 m transect.If the plant was not present, the cell has a 0;if the plant was present in every quarter-meter-squared quadrat, the number in the cell is 20.

ItemTransect 1Transect 2FamilyScientific NameCommon Name
PeltVirg115AraceaePeltandra virginicaGreen arum, Green arrow arum
BlecSerr60BlechnaceaeBlechnum serrulatumSwamp fern, Toothed midsorus fern
CladJama201CyperaceaeCladium jamaicenseSaw-grass, Jamaica swamp sawgrass
EleoCell018CyperaceaeEleocharis cellulosaGulf Coast spikerush
RhynMicr03CyperaceaeRhynchospora microcarpaSouthern beaksedge
UtriFoli03LentibulariaceaeUtricularia foliosaLeafy bladderwort
UtriRadi018LentibulariaceaeUtricularia radiataLittle floating bladderwort
NympAqua02MenyanthaceaeNymphoides aquaticaBig floatingheart
NympOdor014NymphaeaceaeNymphaea odorataAmerican white waterlily
PaniHemi018PoaceaePanicum hemitomonMaidencane
LPX

Exotics

In the 2005 R-EMAP sampling invasive exotic plant species were surveyed in two ways. The first survey was done on the helicopter fly-in to the GPS site and covered the general area of the site, while the second was a 360° site survey from the helicopter pontoons after landing and covered the immediate area of the site. In the aerial (A) helicopter survey we looked for the presence of six invasive exotic species (Casuarina spp., Lygodium microphyllum, Melaleuca quinquenervia, Neyraudia reynaudiana, Pennisetum purpureum and Schinus terebinthefolius), as well as the presence of cattail (Typha domingensis). In the survey from the helicopter floats (F) we recorded the presence of any exotic seen; the only additional exotic species observed from the floats was Salvinia minima. In both surveys we recorded the presence of species and quantified their abundance using categories for the number of individuals. The categories used were 0 (i.e., not present), 1 individual, 2 - 10 individuals, and > 10. We did not find Neyraudia reynaudiana at any site, so this species was not included in the database.

ItemAbundanceScientific NameSource
CasuSp_A0Casuarina speciesaerial fly-in
CasuSp_F0Casuarina speciesfloats
LygoMicr_A> 10Lygodium microphyllumaerial fly-in
LygoMicr_F0Lygodium microphyllumfloats
MelaQuin_A> 10Melaleuca quinquenerviaaerial fly-in
MelaQuin_F0Melaleuca quinquenerviafloats
PennPurp_A0Pennisetum purpureumaerial fly-in
PennPurp_F0Pennisetum purpureumfloats
SalvMini_F0Salvinia minimafloats
SchiTere_A0Schinus terebinthefoliusaerial fly-in
TyphDomi_A0Typha domingensisaerial fly-in
TyphDomi_F0Typha domingensisfloats

Vegetation Map

Aquatic

As part of the EPA‘s R-EMAP Phase III wet season sampling, 54 sites (designated by Olsen numbers/station numbers) spanning the Greater Everglades were sampled for aquatic fauna in November and December 2005. Densities of small fish and large macroinvertebrates were quanitifed using a standardized throw-trap methodology, and macroinvertebrate infauna were enumerated from periphyton cores and floc samples. Community compositions and wet weights were recorded for throw-trap samples, and fish and crayfish lengths were measured. Counts and community compositions were recorded for infauna and scaled according to substrate samples (g AFDM and/or mL of periphyton or floc). Finally, stable isotope analysis was conducted on a subset of the fauna. These data were then used to address questions about consumer densities, community structure, and food webs along hydrological (disturbance) and productivity gradients.

Three throw-traps replicates were deployed per station.

TypeItemThrowtrap 1Throwtrap 2Throwtrap 3UnitsScientific NameCommon NameNote
PeriCover5510%percent of trap area covered by periphyton
PeriVolume12008001300mlbiovolume of periphyton and submerged plants
PlantCover352530%percent of water surface covered by vegetation
PlantHt232117cmplant height from water surface
WaterDepth504950cm
Fish0TotalFish225340
Fishelaeve103Elassoma evergladeiEverglades pygmy sunfish
Fishfunchr304Fundulus chrysotusGolden topminnow
Fishgamhol51311Gambusia holbrookiMosquitofish
Fishhetfor133721Heterandria formosaLeast killifish
Fishjorflo010Jordanella floridaeFlagfish
Fishlucgoo021Lucania goodeiBluefin killifish
Invertebrates0TotalInv153519
Inverterbatesacrgry001Acris gryllusCricket frog adult
Inverterbatescelepo111Celithemis eponinaDragonfly larva
Inverterbatescoenag010family CoenagrionidaeDamselfly larvae
Inverterbatescybspp001Cybister spp.Beetle larva
Inverterbatesepheme010order EphemeropteraMayfly larvae
Inverterbateslibnee100Libellula needhamiDragonfly larva
Inverterbatespalpal71911Palaemonetes paludosusGrass shrimp
Inverterbatespelfem030Pelocoris femoratusAlligator flea
InverterbatesprofalF340Procambarus fallax FCrayfish (female)
InverterbatesprofalM233Procambarus fallax MCrayfish (male)
Inverterbatesprospp002Procambarus sppCrayfish unknown species
Inverterbatesranatp130Rana spp. tp.Pig frog tadpole/Leopard frog tadpole
Vegetationbaccar100percent of floating mat volumeBacopa carolinianawater hyssop
Vegetationclajam300stem countsCladium jamaicensesaw-grass
Vegetationelecel28208stem countsEleocharis cellulosaSpikerush
Vegetationeleelo17271143stem countsEleocharis elongataSpikerush
Vegetationnymodo265stem countsNymphaea odoratafragrant water-lily
Vegetationpanhem71811stem countsPanicum hemitomonmaidencane
Vegetationponcor003stem countsPontederia cordatapickerelweed
Vegetationutrifol0700percent of floating mat volumeUtricularia foliosabright green bladderwort
Vegetationutrpur952595stem countsUtricularia purpureapurple bladderwort